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Tissue Clearing SmartSPIM LifeCanvas, USA

Light sheet imaging system optimized for resolution, speed, and flexibility for large samples

 

SmartSPIM is an advanced light sheet microscope capable of imaging large tissue samples with high speed and resolution. The system was designed from the ground up, with no limitation based on commercially available microscope bodies. The unique illumination arm of the SmartSPIM creates a flat-top illumination profile laterally, and a focused beam axially. 

To achieve homogeneous axial point spread functions (PSFz) across the entire FOV, the illumination optics scan the beam axially while synchronized to the camera’s rolling shutter detection.

 

þ Features 

• Ability to use custom and commercially available objective

• Custom illumination optics Zemax designed and optimized for achromatic performance across all points in the sweeping field of view

• Detection optics Zemax designed and optimized for all current large format objectives and camera sensors

• Focus compensation for RI mismatches and chromatic focal shifts

• Compatible with both aqueous and organic clearing methods

• Simple, user-friendly software interface

• High speed acquisition: 20-40 minutes per channel per organ at single cell resolution, enabling the acquisition of dozens of datasets in a single week


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