New high-throughput system for rapid active tissue clearing and immunolabeling

SmartBatch+ combines active tissue clearing, leveraging our new Clear+ system, and active immunolabeling into one turn-key batch tissue processing device! Now you can clear and label up to 12 whole mouse brains in just one day.

 

An advance on our founder’s CLARITY method, Clear+ tissue clearing provides maximum optical transparency with no tissue expansion; eFLASH and patented SE technologies then uniformly label whole organs from surface to core.

þ Features

• Multi-functional: Switches between clearing and labeling modes simply by changing buffers.

• Fast, uniform labeling: Our system uses eFLASH technology(bioRxiv 2019), combining SWITCH (Cell 2015) with our patented stochastic electrotransport (SE, PNAS 2015) method to uniformly label intact tissue volumes.

• Cost-effective: SmartBatch+ uses just 4-20 μg of antibody per target to label a sample the size of a whole mouse brain, and requires <12 mins hands-on time per sample.

• High-throughput: Our device can actively clear or label up to 12 whole mouse brains, 2 whole rat brains, or comparably sized samples per batch.

•  Ideal for light sheet imaging: Combined with SHIELD tissue preservation, SmartBatch+ protects fluorescent protein signal,    antigenicity, and molecular & physical architecture through all tissue processing steps.